| Gene | Cxx1c |
| Chromosome | X |
| Deletion Start | 53,608,049 |
| Deletion End | 53,608,540 |
| Deletion Size | 492 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,608,174 | 53,608,192 | CGTGCAGACCCTGTCCTAC |
| TUP | 53,608,198 | 53,608,226 | AGTAGATGAGAAGACCTTCGACACCGACA |
| TUR | 53,608,244 | 53,608,262 | CCTTGAGGCGGGTGATGAG |
| TDF | 53,608,362 | 53,608,381 | GGGAGGAGGATGAGGACTTC |
| TDP | 53,608,382 | 53,608,402 | TAGGCCTCTGGCAAGCCTGGC |
| TDR | 53,608,418 | 53,608,437 | GGTAGCAGCTCACCCTCTTG |
PCR Primers
| Primer | Sequence |
| SU | GCTAGGAGTCCAGCATCCTC |
| SD | GAAGGTGAATCAATGTCCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12888A-A3 | VGB6 | C57BL/6NTac | |
| 12888B-C2 | VGB6 | C57BL/6NTac | |
| 12888B-C10 | VGB6 | C57BL/6NTac | |
| 12888B-E9 | VGB6 | C57BL/6NTac | |
