| Gene | Rap2b |
| Chromosome | 3 |
| Deletion Start | 61,365,060 |
| Deletion End | 61,365,657 |
| Deletion Size | 598 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,365,238 | 61,365,257 | CGGAGCAGTTCGCCTCAATG |
| TUP | 61,365,258 | 61,365,283 | CGGGACCTGTACATCAAGAATGGCCA |
| TUR | 61,365,290 | 61,365,311 | GACCAGGCTGTAGACGAGAATG |
| TDF | 61,365,411 | 61,365,431 | GACTTGGAGGGTGAACGTGAG |
| TDP | 61,365,434 | 61,365,457 | CTCCTATGGCGAGGGTAAGGCCCT |
| TDR | 61,365,478 | 61,365,497 | GGCCGATGTCTCCATGAAGG |
PCR Primers
| Primer | Sequence |
| SU | ACTCATGCCACGCGTGTCT |
| SD | CCTTCACTTCCTCTGCTTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12890A-B6 | VGB6 | C57BL/6NTac | |
| 12890A-D9 | VGB6 | C57BL/6NTac | |
| 12890A-E10 | VGB6 | C57BL/6NTac | |
| 12890A-H11 | VGB6 | C57BL/6NTac | |
