| Gene | 4921501E09Rik |
| Chromosome | 17 |
| Deletion Start | 33,067,823 |
| Deletion End | 33,065,079 |
| Deletion Size | 2,745 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,067,616 | 33,067,634 | TGGCATGGATCGCCTAGAG |
| TUP | 33,067,582 | 33,067,611 | TGATGCTCTCAAGAGGAAGCCATTGATGAC |
| TUR | 33,067,545 | 33,067,569 | CCTACCCTGGATTTGGTGAATAGAG |
| TDF | 33,065,755 | 33,065,778 | GAATGGAAGTGAATCTGGTGGAAC |
| TDP | 33,065,723 | 33,065,751 | TGATCCACTAAAGGCCAAGAAACAGGTGG |
| TDR | 33,065,675 | 33,065,696 | TGCTGGAAGAGTTTGGGTCATC |
PCR Primers
| Primer | Sequence |
| SU | GTCTCTTTCCAGGCTCCTCC |
| SD | AGAAATTCCTAGGCCCAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12891A-C8 | VGB6 | C57BL/6NTac | |
| 12891A-E10 | VGB6 | C57BL/6NTac | |
| 12891A-F8 | VGB6 | C57BL/6NTac | |
| 12891A-H8 | VGB6 | C57BL/6NTac | |
