| Gene | 4930521A18Rik |
| Chromosome | 1 |
| Deletion Start | 31,222,942 |
| Deletion End | 31,224,461 |
| Deletion Size | 1,520 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,223,039 | 31,223,060 | CCTGGTCCCTTCAAGCACTATC |
| TUP | 31,223,064 | 31,223,091 | TCTCCTCAATCCCGAAGAAGAGGATGGT |
| TUR | 31,223,095 | 31,223,117 | GAGCATTGCCCCTGTTCAAAATC |
| TDF | 31,223,769 | 31,223,793 | GGTTGTTTCATACAATAAGGTGCTG |
| TDP | 31,223,795 | 31,223,814 | TCCATTTCTATAGTGAGAGC |
| TDR | 31,223,861 | 31,223,882 | GGTCATAGCTCTGCTGTGTGAT |
PCR Primers
| Primer | Sequence |
| SU | ATTGGCTGCTGCTGGTATTG |
| SD | TTGATGGTCAACAGTCCTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12892A-A3 | VGB6 | C57BL/6NTac | |
| 12892A-F5 | VGB6 | C57BL/6NTac | |
| 12892A-H4 | VGB6 | C57BL/6NTac | |
