| Gene | Ddit4 |
| Chromosome | 10 |
| Deletion Start | 59,951,509 |
| Deletion End | 59,950,453 |
| Deletion Size | 1,057 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,951,390 | 59,951,409 | CCAGAGAAGAGGGCCTTGAC |
| TUP | 59,951,351 | 59,951,373 | AGAGCTCGGACTGCGAGTCCCTG |
| TUR | 59,951,331 | 59,951,350 | AAGCCACTGTTGCTGCTGTC |
| TDF | 59,950,710 | 59,950,728 | GGTGCCCACCTTTCAGTTG |
| TDP | 59,950,685 | 59,950,707 | CCTGGTGCTGCGTCTGGACTCTC |
| TDR | 59,950,625 | 59,950,644 | AACCAGGGACCAAGGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | GAGAGGGTTCGAAGGGACAG |
| SD | TACACAAACTGTCGGCCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12893A-A4 | VGB6 | C57BL/6NTac | |
| 12893A-A12 | VGB6 | C57BL/6NTac | |
| 12893A-D6 | VGB6 | C57BL/6NTac | |
| 12893A-H8 | VGB6 | C57BL/6NTac | |
