| Gene | 4930563D23Rik |
| Chromosome | 16 |
| Deletion Start | 92,321,395 |
| Deletion End | 92,320,540 |
| Deletion Size | 856 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,321,043 | 92,321,062 | AAGAGGGCCTATGGCAAGTG |
| TUP | 92,321,020 | 92,321,042 | TGCCATCGTGGTCAAAGGGAGAA |
| TUR | 92,320,989 | 92,321,011 | TCTGTCTTTGGCTTCATCTCATC |
| TDF | 92,320,692 | 92,320,714 | CTGACCAAGTGGAAGGCCATAAC |
| TDP | 92,320,663 | 92,320,691 | CAACAAGGTGCGCAGGTGGGAGAACTATT |
| TDR | 92,320,618 | 92,320,638 | TCAGGGCTTTGTGGAACAGAC |
PCR Primers
| Primer | Sequence |
| SU | GCACAATGTCTGATTCACGG |
| SD | CACGTGTCTCTTGCTCATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12894A-B10 | VGB6 | C57BL/6NTac | |
| 12894A-D4 | VGB6 | C57BL/6NTac | |
| 12894A-F3 | VGB6 | C57BL/6NTac | |
| 12894A-G5 | VGB6 | C57BL/6NTac | |
