| Gene | Lin37 |
| Chromosome | 7 |
| Deletion Start | 30,559,472 |
| Deletion End | 30,555,468 |
| Deletion Size | 4,005 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,559,257 | 30,559,279 | TGTCCCTGAATCAATCCTCACTG |
| TUP | 30,559,226 | 30,559,254 | TGTGCATTCCAATAACGCTAGGGTGTAGG |
| TUR | 30,559,173 | 30,559,190 | TGCCCGCTTCCTTCCTAC |
| TDF | 30,555,647 | 30,555,664 | CTGCTTGTCCCAGTGTCC |
| TDP | 30,555,617 | 30,555,644 | TGTAGCAGTTGCTAAATCCTCCCTCTGC |
| TDR | 30,555,576 | 30,555,597 | CGAAGCTGGTTCCGATGAGATG |
PCR Primers
| Primer | Sequence |
| SU | GGCCAGCAAATGAAATTGAC |
| SD | ACATCCAAGCCTAGTCCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12895A-D10 | VGB6 | C57BL/6NTac | |
| 12895A-E6 | VGB6 | C57BL/6NTac | |
| 12895A-C6 | VGB6 | C57BL/6NTac | |
| 12895A-B9 | VGB6 | C57BL/6NTac | |
| 12895A-A3 | VGB6 | C57BL/6NTac | |
