| Gene | 5830403L16Rik |
| Chromosome | 1 |
| Deletion Start | 153,851,123 |
| Deletion End | 153,850,313 |
| Deletion Size | 811 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 153,851,012 | 153,851,035 | GCAACTCTCCTGTCCAATATCCAC |
| TUP | 153,850,988 | 153,851,009 | TCCCGTCCTTGGAGCAAAGGGA |
| TUR | 153,850,968 | 153,850,987 | TTGCCGTAACCTTGTCCATC |
| TDF | 153,850,437 | 153,850,454 | GCGTTCGCCACCACTTTC |
| TDP | 153,850,415 | 153,850,436 | TTCTGCTGGCACGTGGTCTCCG |
| TDR | 153,850,387 | 153,850,407 | CCGTAGGCCGAGATCATCAAG |
PCR Primers
| Primer | Sequence |
| SU | GTCCAAGAACAGATGGGCAG |
| SD | CAAGGACAAGAAAGGATGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12897A-E5 | VGB6 | C57BL/6NTac | |
| 12897A-F8 | VGB6 | C57BL/6NTac | |
| 12897A-D1 | VGB6 | C57BL/6NTac | |
