| Gene | Krtap17-1 |
| Chromosome | 11 |
| Deletion Start | 99,993,936 |
| Deletion End | 99,993,543 |
| Deletion Size | 394 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,993,880 | 99,993,900 | CAGGAACAGGACTGCTGTGAG |
| TUP | 99,993,858 | 99,993,878 | AGTGTTGCTGCCAGCAGGGCT |
| TUR | 99,993,797 | 99,993,815 | CCTCCGCAGCTAGAACCTC |
| TDF | 99,993,644 | 99,993,662 | GGTGCTGTGGTCCAGTGTG |
| TDP | | | TGTCAGCCCACACC |
| TDR | 99,993,600 | 99,993,623 | GGGAAGGTCTTCATTTTGTCTCAC |
PCR Primers
| Primer | Sequence |
| SU | AAACAGGCTACATGGCCAAC |
| SD | ATGGACCAAGAGGTCCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12902A-H5 | VGB6 | C57BL/6NTac | |
| 12902A-C7 | VGB6 | C57BL/6NTac | |
| 12902A-G6 | VGB6 | C57BL/6NTac | |
| 12902A-B2 | VGB6 | C57BL/6NTac | |
