| Gene | P2ry4 |
| Chromosome | X |
| Deletion Start | 100,594,287 |
| Deletion End | 100,593,178 |
| Deletion Size | 1,110 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,593,890 | 100,593,909 | CACCGCTACATGGGCATCTG |
| TUP | 100,593,867 | 100,593,889 | CCATCCACTGCGGGCAATACGCT |
| TUR | 100,593,806 | 100,593,828 | CAGCCAGCTACTACCAACCAAAC |
| TDF | 100,593,311 | 100,593,330 | ACACCTAAGCGCCGAACAAC |
| TDP | 100,593,288 | 100,593,310 | TGCTTCCTCCCTGGCATTGGTGA |
| TDR | 100,593,235 | 100,593,255 | GATGCTGTCTTGGTGGATGTC |
PCR Primers
| Primer | Sequence |
| SU | GAGGTGAGGGAAAGAGGTCC |
| SD | TCCTGGTTCTGCCTTGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12903A-E5 | VGB6 | C57BL/6NTac | |
| 12903A-F3 | VGB6 | C57BL/6NTac | |
