| Gene | Foxb1 |
| Chromosome | 9 |
| Deletion Start | 69,760,243 |
| Deletion End | 69,759,229 |
| Deletion Size | 1,015 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,760,208 | 69,760,229 | CAACACGTACAGCGACCAGAAG |
| TUP | 69,760,180 | 69,760,206 | CGCCCTACTCATACATCTCGCTGACCG |
| TUR | 69,760,106 | 69,760,127 | GAAGCGGTCCATGATGAACTTG |
| TDF | 69,759,638 | 69,759,658 | GCCTATCCTCTCCCTAACCAG |
| TDP | 69,759,611 | 69,759,632 | TACCATGGGCAGCTCTCTGGGC |
| TDR | 69,759,559 | 69,759,579 | GGGTGGCTGAGTCGATCATAC |
PCR Primers
| Primer | Sequence |
| SU | AAACCTCTCTGCTGCTCTCG |
| SD | GTCCAGAGCTATGCCTCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 124 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12904A-D6 | VGB6 | C57BL/6NTac | |
| 12904A-F4 | VGB6 | C57BL/6NTac | |
| 12904A-F5 | VGB6 | C57BL/6NTac | |
| 12904A-G1 | VGB6 | C57BL/6NTac | |
