| Gene | Htra2 |
| Chromosome | 6 |
| Deletion Start | 83,054,423 |
| Deletion End | 83,051,402 |
| Deletion Size | 3,022 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,053,769 | 83,053,792 | CCCATCTCAAACGGATCAGGATTC |
| TUP | 83,053,739 | 83,053,764 | TGGCTTCAGATGGGCTCATCGTTACC |
| TUR | 83,053,692 | 83,053,710 | GGCAGCCTCACTCGTACTC |
| TDF | 83,052,142 | 83,052,159 | AGCACTGGAGGCCCATTC |
| TDP | 83,052,117 | 83,052,140 | CGCCCATTCCCACCCATCTCGTTA |
| TDR | 83,052,089 | 83,052,108 | CGAAGAAACGGCAAGCTGTC |
PCR Primers
| Primer | Sequence |
| SU | CTTACCGGTGCGAGTCAAAG |
| SD | ACAAGCATGGAGAATCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12905A-C1 | VGB6 | C57BL/6NTac | |
| 12905A-C11 | VGB6 | C57BL/6NTac | |
| 12905A-H9 | VGB6 | C57BL/6NTac | |
