12906   Ccl24     Status: ES cell colonies screened / QC positives

GeneCcl24
Chromosome5
Deletion Start135,572,969
Deletion End135,570,788
Deletion Size2,182
Genome Build38
MGI IDMGI:1928953
NCBI Gene ID56221
BAC ID302D5
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,572,907135,572,925CTGCGCGTGTTGCATCTTC
TUP135,572,883135,572,906CCCATAGGTGAGACTTCTGGGCCA
TUR135,572,821135,572,840CACCCTTCACTGTGCCAAAC
TDF135,571,011135,571,035TCCTCATAAAGGGAAGGTTAGTCTC
TDP135,570,983135,571,010CACACCTGGCAGGACGTCAAACTAAACC
TDR135,570,952135,570,974TTGGTGATGAAGCTGTAGGAAAG

PCR Primers

PrimerSequence
SUATGAGTGGACAGAAGGCAGG
SDAGAACCCTATGGAAGCCAGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR105 bp++-
TDFTDR84 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12906C-B4VGB6C57BL/6NTac
12906C-F4VGB6C57BL/6NTac
12906C-D6VGB6C57BL/6NTac
12906C-D9VGB6C57BL/6NTac
12906C-D4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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