| Gene | Cst10 |
| Chromosome | 2 |
| Deletion Start | 149,405,369 |
| Deletion End | 149,410,060 |
| Deletion Size | 4,692 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 149,405,425 | 149,405,444 | CCTGGCTGTGATCCCTGAAG |
| TUP | 149,405,445 | 149,405,473 | CCAGTACTAATGCTGAAGCCAAGCAGGTG |
| TUR | 149,405,474 | 149,405,494 | TGGCTCCACTCCACCTAATAC |
| TDF | 149,409,930 | 149,409,950 | CCAACACTGACTGTCCCTTTC |
| TDP | 149,409,955 | 149,409,973 | TCTCTTCTTTTGTAGAGGG |
| TDR | 149,410,005 | 149,410,026 | CATGGACATCTTGTTCAGCCAA |
PCR Primers
| Primer | Sequence |
| SU | CCAAGGTGACCTGGAGAGAG |
| SD | CCATTGTACATTCCTATGTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12907A-G1 | VGB6 | C57BL/6NTac | |
| 12907A-A10 | VGB6 | C57BL/6NTac | |
| 12907A-B6 | VGB6 | C57BL/6NTac | |
| 12907A-E9 | VGB6 | C57BL/6NTac | |
| 12907A-A2 | VGB6 | C57BL/6NTac | |
