| Gene | Papolb |
| Chromosome | 5 |
| Deletion Start | 142,529,883 |
| Deletion End | 142,527,856 |
| Deletion Size | 2,028 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 142,529,768 | 142,529,786 | ACACAGACCGCGAACTCAC |
| TUP | 142,529,744 | 142,529,767 | CCAGAAGCTGATCGAGACCCTCCA |
| TUR | 142,529,712 | 142,529,734 | GTTCCTCTTCCTCTTCAAACACC |
| TDF | 142,528,217 | 142,528,237 | AAACCCTCAGGGCAGAAACAG |
| TDP | 142,528,190 | 142,528,214 | CTGCTCTGGCTGTTATGGCAGCATC |
| TDR | 142,528,154 | 142,528,174 | TGCAAGGAAACATCAGGAAAC |
PCR Primers
| Primer | Sequence |
| SU | TCCTCCAATCTCGTGAGAGC |
| SD | GGACTTCAAGATTAGGCCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12909B-C7 | VGB6 | C57BL/6NTac | |
| 12909B-E5 | VGB6 | C57BL/6NTac | |
| 12909B-F7 | VGB6 | C57BL/6NTac | |
| 12909B-G8 | VGB6 | C57BL/6NTac | |
| 12909B-F2 | VGB6 | C57BL/6NTac | |
