| Gene | Cstf2t |
| Chromosome | 19 |
| Deletion Start | 31,083,069 |
| Deletion End | 31,085,008 |
| Deletion Size | 1,940 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,083,224 | 31,083,243 | TGGGAAGCCCAAGGGTTATG |
| TUP | 31,083,246 | 31,083,271 | TTCTGCGAGTACCAAGACCAGGAGAC |
| TUR | 31,083,276 | 31,083,296 | GAGGTTTCGCATGGCACTGAG |
| TDF | 31,084,055 | 31,084,074 | GCCTTCTGGTGGCATACCTC |
| TDP | 31,084,075 | 31,084,100 | CTCGAGGACTACTGGGAGATGCTCCA |
| TDR | 31,084,118 | 31,084,139 | TCCAGTCACTGAGAGCAAAGTC |
PCR Primers
| Primer | Sequence |
| SU | GTGTGTGGAAACACAGACGC |
| SD | TAGTTCCCAGCTTACTCGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12910A-A2 | VGB6 | C57BL/6NTac | |
| 12910A-E1 | VGB6 | C57BL/6NTac | |
