Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

12911 Gpr87 Status: ES cell colonies screened / QC positives

Gene	Gpr87
Chromosome	3
Deletion Start	59,180,082
Deletion End	59,178,934
Deletion Size	1,149
Genome Build	38

MGI ID	MGI:1934133
NCBI Gene ID	84111
BAC ID	219F14
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 3

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	59,180,003	59,180,022	CACACTGCAAACAGCACAAG
TUP	59,179,977	59,180,002	TGAGGGACATGGGAAGAACTCTACCC
TUR	59,179,936	59,179,957	GTAAAGCACTGGCAGGATGATG
TDF	59,179,067	59,179,087	AACCAGGAGCGAAAGCATCAG
TDP	59,179,042	59,179,064	CGCTGCAAAGCGTCCGAAGATCA
TDR	59,178,993	59,179,014	TGGCCTCTAGTCCTTACACATC

PCR Primers

Primer	Sequence
SU	TTCTTTAAATCTTGGCAGCAG
SD	TCTTAGCCTTGCTCCCTCTG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	87 bp	+	+	-
TDF	TDR	95 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
12911A-A5	VGB6	C57BL/6NTac
12911A-C5	VGB6	C57BL/6NTac
12911A-D6	VGB6	C57BL/6NTac
12911A-G3	VGB6	C57BL/6NTac