| Gene | Mrgprh |
| Chromosome | 17 |
| Deletion Start | 12,876,878 |
| Deletion End | 12,877,907 |
| Deletion Size | 1,030 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 12,877,237 | 12,877,255 | CACGGCCATCAGCGTAGAG |
| TUP | 12,877,256 | 12,877,279 | CGGTGCCTCTCCGTGCTTTATCCA |
| TUR | 12,877,324 | 12,877,342 | GGCCCACAGCAGCATACAG |
| TDF | 12,877,558 | 12,877,579 | CACGGTCATCCTGTTTCTTGTC |
| TDP | 12,877,582 | 12,877,606 | CGCCATGCCCATGAAGGTGTTGCTT |
| TDR | 12,877,634 | 12,877,658 | GGAGGGAGTCCCATACAGAATCATC |
PCR Primers
| Primer | Sequence |
| SU | GAGAGCTTTCCCAGAGAGCC |
| SD | ACGAAGTGCGACAGTGTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12912A-D3 | VGB6 | C57BL/6NTac | |
| 12912A-F6 | VGB6 | C57BL/6NTac | |
| 12912A-F8 | VGB6 | C57BL/6NTac | |
| 12912A-H10 | VGB6 | C57BL/6NTac | |
