| Gene | Hes7 |
| Chromosome | 11 |
| Deletion Start | 69,120,477 |
| Deletion End | 69,123,032 |
| Deletion Size | 2,556 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,120,968 | 69,120,988 | AAAGGCTATGGACGCTGTCTG |
| TUP | 69,120,990 | 69,121,011 | AAGCGCCTGGGAACCCACATCT |
| TUR | 69,121,020 | 69,121,041 | CCAACGACTCAAAGGTCTTCTC |
| TDF | 69,122,917 | 69,122,937 | CCACCGCCTTACAGACAAGAC |
| TDP | 69,122,942 | 69,122,961 | CGCCCAAGGCCCCGTCACTC |
| TDR | 69,122,981 | 69,123,000 | AAAAGCTCAGGGCCAAGGTC |
PCR Primers
| Primer | Sequence |
| SU | TCTGCTCTGCACCTTTAGGG |
| SD | AAGGTGGGAGAAGGGTATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12913A-D10 | VGB6 | C57BL/6NTac | |
| 12913A-E6 | VGB6 | C57BL/6NTac | |
| 12913A-F6 | VGB6 | C57BL/6NTac | |
| 12913A-A7 | VGB6 | C57BL/6NTac | |
