| Gene | B630019K06Rik |
| Chromosome | X |
| Deletion Start | 8,892,761 |
| Deletion End | 8,893,843 |
| Deletion Size | 1,083 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,892,789 | 8,892,807 | ACCGTGCGAGCCAGAAGAA |
| TUP | 8,892,817 | 8,892,835 | CTGCAGCTGGTGTCGCCGC |
| TUR | | | GCCCGGCAGCCTGAT |
| TDF | 8,893,543 | 8,893,561 | TCCTGCCTCAGAGTGTTCC |
| TDP | 8,893,562 | 8,893,583 | TTCATTGAAGCCACCGGCGACA |
| TDR | 8,893,584 | 8,893,602 | ATGCTTCCAGCCCGGAGAG |
PCR Primers
| Primer | Sequence |
| SU | TCTTGGAGTGGGTGGTGTG |
| SD | GGCACTAACTAGCAGCGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TDF | TDR | 60 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12915A-E7 | VGB6 | C57BL/6NTac | |
| 12915A-F3 | VGB6 | C57BL/6NTac | |
