| Gene | Magee1 |
| Chromosome | X |
| Deletion Start | 105,120,614 |
| Deletion End | 105,123,379 |
| Deletion Size | 2,766 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,121,001 | 105,121,019 | CCGCCCACTGCTCCTAAAG |
| TUP | 105,121,022 | 105,121,048 | TCAAAGGCCTATGAGCATCTCCCTGTC |
| TUR | 105,121,063 | 105,121,083 | GAGTGACGCTGCTCAGAGATG |
| TDF | 105,122,832 | 105,122,852 | CCAAAGTGTTCCCTGATCTCC |
| TDP | 105,122,853 | 105,122,874 | TGAATCGTGCTGCTCGCACCCT |
| TDR | 105,122,884 | 105,122,905 | GACCACTAGCTCTGTGCCATAC |
PCR Primers
| Primer | Sequence |
| SU | AATCACCAGGTCCACTTTCG |
| SD | GGAAGCCAGCCACAACTTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12916B-A7 | VGB6 | C57BL/6NTac | |
| 12916B-A10 | VGB6 | C57BL/6NTac | |
| 12916B-D4 | VGB6 | C57BL/6NTac | |
| 12916B-G10 | VGB6 | C57BL/6NTac | |
