| Gene | Rac3 |
| Chromosome | 11 |
| Deletion Start | 120,721,610 |
| Deletion End | 120,723,672 |
| Deletion Size | 2,063 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,722,340 | 120,722,358 | CACTAGAGGCCAGGCACAC |
| TUP | 120,722,360 | 120,722,382 | TGCATGGGACCTCCCAAGGTTCC |
| TUR | 120,722,405 | 120,722,424 | TCACCACGGAAACCCAGAAC |
| TDF | 120,723,010 | 120,723,029 | TCCCTGGGTGGGTATCTTTG |
| TDP | 120,723,033 | 120,723,060 | ACTGCCAAACCCTACAGACTCCTAGTCA |
| TDR | 120,723,063 | 120,723,083 | TTGGCTCTTCCCGCTTTACAG |
PCR Primers
| Primer | Sequence |
| SU | GTAGTGGGTCGTAGACACG |
| SD | AGAAAGTAGGGCAGACAGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12920A-A2 | VGB6 | C57BL/6NTac | |
| 12920A-C4 | VGB6 | C57BL/6NTac | |
| 12920A-E8 | VGB6 | C57BL/6NTac | |
| 12920A-H7 | VGB6 | C57BL/6NTac | |
