| Gene | Rxfp4 |
| Chromosome | 3 |
| Deletion Start | 88,653,139 |
| Deletion End | 88,651,821 |
| Deletion Size | 1,319 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,653,038 | 88,653,055 | GCCATGCCTGTCCAGTTC |
| TUP | 88,653,008 | 88,653,031 | CTGAGGATCATGGTTGCACTGGCC |
| TUR | 88,652,980 | 88,653,003 | GCAAGCCAATGATACCTACAAGTC |
| TDF | 88,652,247 | 88,652,269 | ACTTACATCCTTCCCATCACCAC |
| TDP | 88,652,223 | 88,652,246 | CTGCTTGGCCCACAGCAACAGCTG |
| TDR | 88,652,192 | 88,652,214 | CCGCAGGAGACAATAGATCACAG |
PCR Primers
| Primer | Sequence |
| SU | GTCTTCTGGTTTGGCTCCAC |
| SD | CTCAGGGTCTGCATCTTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12921A-C3 | VGB6 | C57BL/6NTac | |
| 12921A-H11 | VGB6 | C57BL/6NTac | |
| 12921A-F2 | VGB6 | C57BL/6NTac | |
| 12921A-H2 | VGB6 | C57BL/6NTac | |
| 12921A-G1 | VGB6 | C57BL/6NTac | |
