12922   Uts2r     Status: Germline transmission achieved

GeneUts2r
Chromosome11
Deletion Start121,160,315
Deletion End121,161,492
Deletion Size1,178
Genome Build38
MGI IDMGI:2183450
NCBI Gene ID217369
BAC ID304I14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF121,160,377121,160,398GCTTCCTGGTGGCTTTAATGTG
TUP121,160,399121,160,422TCCCACAACAGTTCCTGGACTGGC
TUR121,160,424121,160,443CAGGGAGCTGGGATCTGTTG
TDF121,161,186121,161,205AGACTGCACGCATCATCAAC
TDP121,161,207121,161,231ACCTGACTGCCTGCCTCACTTACGG
TDR121,161,244121,161,266GCAGAGTGTAGAGGAAGGGATTG

PCR Primers

PrimerSequence
SUGTTCTGCTGGAGGAGTGTCC
SDTGAGGCAGGACACACTTCAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR67 bp++-
TDFTDR81 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12922A-B6VGB6C57BL/6NTac
12922A-F11VGB6C57BL/6NTac
12922A-F12VGB6C57BL/6NTac
12922A-H5VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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