| Gene | Gjd3 |
| Chromosome | 11 |
| Deletion Start | 98,983,013 |
| Deletion End | 98,982,094 |
| Deletion Size | 920 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,982,856 | 98,982,875 | GAGCAGGAGGAGTTCGTGTG |
| TUP | 98,982,834 | 98,982,855 | TAACACGTTGCAGCCCGGCTGT |
| TUR | 98,982,762 | 98,982,782 | CAGCAGCAGGATGTGGAAGAG |
| TDF | 98,982,205 | 98,982,224 | AGCAAAGCGTCGCTGGCTAC |
| TDP | 98,982,179 | 98,982,200 | CGCCAGGACCTGGCCATCTAGC |
| TDR | 98,982,100 | 98,982,120 | GGGTCCTACGCAGTCTTTCAG |
PCR Primers
| Primer | Sequence |
| SU | TGCGAGGTGAGAGAGAAAGG |
| SD | GCCTGTCACTTGGGAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12923A-C7 | VGB6 | C57BL/6NTac | |
| 12923A-G6 | VGB6 | C57BL/6NTac | |
| 12923A-C9 | VGB6 | C57BL/6NTac | |
| 12923A-F7 | VGB6 | C57BL/6NTac | |
