| Gene | Mgat2 |
| Chromosome | 12 |
| Deletion Start | 69,184,657 |
| Deletion End | 69,186,111 |
| Deletion Size | 1,455 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,185,148 | 69,185,166 | CCCGGTTCTGCAAGTGTTC |
| TUP | 69,185,169 | 69,185,193 | TCCGTTCAGCATTCAGCTGTACCCG |
| TUR | 69,185,197 | 69,185,216 | GGGTCACTACCCGGAAACTC |
| TDF | 69,185,570 | 69,185,592 | CTTGGAAATCGACAGAGCACAAC |
| TDP | 69,185,601 | 69,185,626 | AGCCTTGACCCGAGATGCATATCAGA |
| TDR | 69,185,635 | 69,185,655 | ACAGAAAGTGTCCGTGCATTC |
PCR Primers
| Primer | Sequence |
| SU | AGCGCAGGTAAAGGAATCAC |
| SD | GCACAGGAATCAGTAATACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12924A-C5 | VGB6 | C57BL/6NTac | |
| 12924A-F3 | VGB6 | C57BL/6NTac | |
