| Gene | Npb |
| Chromosome | 11 |
| Deletion Start | 120,608,512 |
| Deletion End | 120,608,986 |
| Deletion Size | 475 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,608,623 | 120,608,643 | GGGCTACTGTCGAGTTTCCAC |
| TUP | 120,608,644 | 120,608,665 | AGGTTCCCGTCCACGCGACGCT |
| TUR | 120,608,728 | 120,608,749 | TTGCACTCACAAGGCTCCTTAC |
| TDF | 120,608,867 | 120,608,886 | TCAACAGCCGAGGGACTTTC |
| TDP | 120,608,890 | 120,608,914 | TGTAAAGCGGACGTCTTCTTGTCGC |
| TDR | 120,608,926 | 120,608,945 | GCTCAGGTGCTCTGGCAATC |
PCR Primers
| Primer | Sequence |
| SU | TGGAAGTTGATACATCTGGTCAG |
| SD | AAAGACTGAGCAGCAGGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12926A-D7 | VGB6 | C57BL/6NTac | |
| 12926A-D12 | VGB6 | C57BL/6NTac | |
| 12926A-E9 | VGB6 | C57BL/6NTac | |
| 12926A-F8 | VGB6 | C57BL/6NTac | |
