| Gene | Ng23 |
| Chromosome | 17 |
| Deletion Start | 35,027,907 |
| Deletion End | 35,026,347 |
| Deletion Size | 1,561 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,027,613 | 35,027,631 | GTCCTGCTGGGAGTGAGTC |
| TUP | 35,027,586 | 35,027,609 | TTGGCCTGTGTCTCCTCTAAGCCA |
| TUR | 35,027,561 | 35,027,579 | CTCCAGAGCCTGCATCATC |
| TDF | 35,026,865 | 35,026,886 | GGTTCTGGGAGAAGGGAATCTG |
| TDP | 35,026,798 | 35,026,818 | CCAGCACTCGGTCACCCTCCA |
| TDR | 35,026,751 | 35,026,772 | GGCATCAGTCTCTGAATGCAAG |
PCR Primers
| Primer | Sequence |
| SU | TTACATTTAGTCAGGAATGGTGG |
| SD | CAACAGGAGCCAAACCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 136 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12927A-B11 | VGB6 | C57BL/6NTac | |
| 12927A-C5 | VGB6 | C57BL/6NTac | |
| 12927A-D7 | VGB6 | C57BL/6NTac | |
| 12927A-F5 | VGB6 | C57BL/6NTac | |
