| Gene | Adam1a |
| Chromosome | 5 |
| Deletion Start | 121,521,225 |
| Deletion End | 121,518,776 |
| Deletion Size | 2,450 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,521,189 | 121,521,207 | AGAGGGTTTGCCTCCAGTC |
| TUP | 121,521,166 | 121,521,188 | TGTCTTCCCCACAGATCAGGCGA |
| TUR | 121,521,124 | 121,521,144 | CGCCCAGATGTGAGGTGTTAG |
| TDF | 121,519,403 | 121,519,425 | AGATACCTGCCCAAAGTCAAACC |
| TDP | 121,519,372 | 121,519,399 | ACACTCACTCCTCCAGGTTCCTTATGGA |
| TDR | 121,519,350 | 121,519,371 | CCATACTCCAACACCAGTCCTC |
PCR Primers
| Primer | Sequence |
| SU | TGAGCCACTGTAGATGCAGG |
| SD | GCCTTAAAACAATAGATCCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12928A-B8 | VGB6 | C57BL/6NTac | |
| 12928A-D4 | VGB6 | C57BL/6NTac | |
| 12928A-F9 | VGB6 | C57BL/6NTac | |
| 12928A-G1 | VGB6 | C57BL/6NTac | |
