12929   Adam1b     Status: ES cell colonies screened / QC positives

GeneAdam1b
Chromosome5
Deletion Start121,502,977
Deletion End121,500,457
Deletion Size2,521
Genome Build38
MGI IDMGI:2429506
NCBI Gene ID280667
BAC ID130F2
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF121,502,934121,502,954CCCAGAGCACTGGTGTATCAG
TUP121,502,907121,502,931TGGTGGCCATGTTGCTCTTGGCAAT
TUR121,502,826121,502,845GGCAGTCTCTCAGGGATGAC
TDF121,501,251121,501,272CCTTCCTGCCACAAATCCAACC
TDP121,501,224121,501,248ACAACTTGCTGCTCCAGGTCCCTGA
TDR121,501,198121,501,219CTACACTCCAGCACCAGTCCTC

PCR Primers

PrimerSequence
SUAGGCACCTCTCTCCATCTTG
SDAAATGGACTCAACACATACATCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR129 bp++-
TDFTDR75 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12929A-F6VGB6C57BL/6NTac
12929A-F7VGB6C57BL/6NTac
12929A-D3VGB6C57BL/6NTac
12929A-G2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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