| Gene | Rbm15 |
| Chromosome | 3 |
| Deletion Start | 107,333,077 |
| Deletion End | 107,326,225 |
| Deletion Size | 6,853 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,332,510 | 107,332,528 | GTGGAGGACGGGCTGTTTC |
| TUP | 107,332,480 | 107,332,508 | CGAGTTCAAACGCTTCGGTGATGTAAGTG |
| TUR | 107,332,424 | 107,332,444 | CACAAAGGCTACCCGCTCATC |
| TDF | 107,327,026 | 107,327,050 | GAGAACAAGAACCAGAGTTAGCCTT |
| TDP | 107,326,977 | 107,327,006 | TGATGCCCTTAACAGTGGGAAATGATTTCA |
| TDR | 107,326,953 | 107,326,975 | GCTTAACCACGTACTGTAGTTGC |
PCR Primers
| Primer | Sequence |
| SU | CGTGCTAGAAGTACCGTTAC |
| SD | TCTGAGCAGCAGCAAGTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12933A-B3 | VGB6 | C57BL/6NTac | |
| 12933A-B5 | VGB6 | C57BL/6NTac | |
| 12933A-B11 | VGB6 | C57BL/6NTac | |
| 12933A-D5 | VGB6 | C57BL/6NTac | |
