| Gene | Sbsn |
| Chromosome | 7 |
| Deletion Start | 30,751,565 |
| Deletion End | 30,756,078 |
| Deletion Size | 4,514 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,751,805 | 30,751,825 | AACGTGGAACATGGCTTGGAC |
| TUP | 30,751,827 | 30,751,853 | AAGTAGCCCACGACATCAACAGTGGCA |
| TUR | 30,751,857 | 30,751,875 | GCTTCCTTTCCTGCGTGTC |
| TDF | 30,755,170 | 30,755,190 | TGGCCTGTTGTCACTCACTTG |
| TDP | 30,755,198 | 30,755,223 | TGGGCACTTTCCAGTCCCAATTAACA |
| TDR | 30,755,256 | 30,755,274 | TGGCTCCCAGATGCTTCAG |
PCR Primers
| Primer | Sequence |
| SU | TCCCTGTCTGCTTTCCACTC |
| SD | ATTGCACAACAGACACAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12938A-F9 | VGB6 | C57BL/6NTac | |
| 12938A-G12 | VGB6 | C57BL/6NTac | |
