Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

12940 Sprr4 Status: ES cell colonies screened / QC positives

Gene	Sprr4
Chromosome	3
Deletion Start	92,500,490
Deletion End	92,500,261
Deletion Size	230
Genome Build	38

MGI ID	MGI:2654508
NCBI Gene ID	229562
BAC ID	135L22
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 3

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	92,500,467	92,500,490	CCTTCCCATCAGCATCAGAATCAG
TUP	92,500,444	92,500,466	CAACAGTGCACACCACAAGCCCA
TUR	92,500,381	92,500,399	GGCACACATGCCTCTTGAC
TDF	92,500,328	92,500,347	AAAACAATGCCCAGCGAGGA
TDP	92,500,294	92,500,319	AACCCAGCTCAAGAGAAGTGTCCTGC
TDR	92,500,270	92,500,290	TGCTTACACTTAGGGTCTTGC

PCR Primers

Primer	Sequence
SU	AGACGCACACCTTGAATTTG
SD	AGAGGCTGGAGCAGTTGAAG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	110 bp	+	+	-
TDF	TDR	78 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
12940A-C11	VGB6	C57BL/6NTac
12940A-B5	VGB6	C57BL/6NTac
12940A-B11	VGB6	C57BL/6NTac
12940A-C6	VGB6	C57BL/6NTac