| Gene | Mafa |
| Chromosome | 15 |
| Deletion Start | 75,747,919 |
| Deletion End | 75,746,791 |
| Deletion Size | 1,129 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,747,581 | 75,747,600 | AAGCGGTGCTGGAGGATCTG |
| TUP | 75,747,557 | 75,747,579 | ACTGGATGAGCGGGTACCAGCAC |
| TUR | 75,747,521 | 75,747,538 | CTCCGGCGTCAGGTTGAG |
| TDF | 75,747,045 | 75,747,067 | CACATTCTGGAGAGCGAGAAGTG |
| TDP | 75,747,024 | 75,747,044 | CCAGCTCCAGAGCCAGGTGGA |
| TDR | 75,746,975 | 75,746,993 | GTACAGGTCCCGCTCCTTG |
PCR Primers
| Primer | Sequence |
| SU | CGGAGGAACAGAAGGAGGA |
| SD | GAGCAAGTAAACGGAGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12941A-A9 | VGB6 | C57BL/6NTac | |
| 12941A-C4 | VGB6 | C57BL/6NTac | |
| 12941A-H6 | VGB6 | C57BL/6NTac | |
| 12941A-H7 | VGB6 | C57BL/6NTac | |
