Gene | Mafa |
Chromosome | 15 |
Deletion Start | 75,747,919 |
Deletion End | 75,746,791 |
Deletion Size | 1,129 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 75,747,581 | 75,747,600 | AAGCGGTGCTGGAGGATCTG |
TUP | 75,747,557 | 75,747,579 | ACTGGATGAGCGGGTACCAGCAC |
TUR | 75,747,521 | 75,747,538 | CTCCGGCGTCAGGTTGAG |
TDF | 75,747,045 | 75,747,067 | CACATTCTGGAGAGCGAGAAGTG |
TDP | 75,747,024 | 75,747,044 | CCAGCTCCAGAGCCAGGTGGA |
TDR | 75,746,975 | 75,746,993 | GTACAGGTCCCGCTCCTTG |
PCR Primers
Primer | Sequence |
SU | CGGAGGAACAGAAGGAGGA |
SD | GAGCAAGTAAACGGAGGCAG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 80 bp | + | + | - |
TDF | TDR | 93 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
12941A-A9 | VGB6 | C57BL/6NTac | |
12941A-C4 | VGB6 | C57BL/6NTac | |
12941A-H6 | VGB6 | C57BL/6NTac | |
12941A-H7 | VGB6 | C57BL/6NTac | |