| Gene | Frat2 |
| Chromosome | 19 |
| Deletion Start | 41,847,908 |
| Deletion End | 41,847,211 |
| Deletion Size | 698 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,847,774 | 41,847,792 | GATCTGGACGATGAGCCGA |
| TUP | 41,847,796 | 41,847,815 | ACGTCGGTCGAGCCGCCCAG |
| TUR | | | CCGGCGACGAGGCA |
| TDF | 41,847,438 | 41,847,458 | GCTTCTCACCGAATCCAGCAG |
| TDP | 41,847,388 | 41,847,406 | CCGACGACGACCCGCACCG |
| TDR | 41,830,777 | 41,830,797 | CGCCTCCTTGATGAGGTTTCC |
PCR Primers
| Primer | Sequence |
| SU | GGAATCGCTCGCAACCATAT |
| SD | CCCACCAACATAAATCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TDF | TDR | 16682 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12942A-F12 | VGB6 | C57BL/6NTac | |
| 12942A-B9 | VGB6 | C57BL/6NTac | |
| 12942A-H6 | VGB6 | C57BL/6NTac | |
