| Gene | Csnka2ip |
| Chromosome | 16 |
| Deletion Start | 64,478,664 |
| Deletion End | 64,477,791 |
| Deletion Size | 874 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,478,523 | 64,478,543 | GCGCAACCCTCTTTGTCATAC |
| TUP | 64,478,500 | 64,478,522 | CAACACCCGAGAGCAGCACCGAT |
| TUR | 64,478,441 | 64,478,463 | GTTGAGGAAGGCTTCTCTGGTAG |
| TDF | 64,478,079 | 64,478,101 | CACTCCTTGGGTAGATCCCACTC |
| TDP | 64,478,050 | 64,478,078 | CGGTTCCGGTTTCAGGATATGTTCTTGCA |
| TDR | 64,478,017 | 64,478,039 | CCCTGGAGGTAACCTGTGATAAC |
PCR Primers
| Primer | Sequence |
| SU | GACCTCGTCAGCTACCATC |
| SD | ATGTTCAGAACCAGGTAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12943A-F8 | VGB6 | C57BL/6NTac | |
| 12943A-H4 | VGB6 | C57BL/6NTac | |
