| Gene | U2af1l4 |
| Chromosome | 7 |
| Deletion Start | 30,563,359 |
| Deletion End | 30,565,246 |
| Deletion Size | 1,888 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,563,944 | 30,563,968 | CAACTTGTACCGGAATCCACAGAAC |
| TUP | 30,563,970 | 30,563,995 | CAGCCCAAACTGCAGATGGATCACAC |
| TUR | 30,564,012 | 30,564,031 | TGCCCAGTCCCTGAACTATC |
| TDF | 30,564,650 | 30,564,673 | GCTTGAGTGTTTGATAGGGAGTTC |
| TDP | 30,564,678 | 30,564,704 | TGATGGTGGCCTTCTCTGTCTTTAGGG |
| TDR | 30,564,727 | 30,564,748 | GGGCCGTAGGTGCATAAAGTTG |
PCR Primers
| Primer | Sequence |
| SU | AAGACCCAAATGACAGACGC |
| SD | GTGGGTTTCAATTACCAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12944A-C4 | VGB6 | C57BL/6NTac | |
| 12944A-C9 | VGB6 | C57BL/6NTac | |
| 12944A-D3 | VGB6 | C57BL/6NTac | |
| 12944A-D8 | VGB6 | C57BL/6NTac | |
