| Gene | Elane |
| Chromosome | 10 |
| Deletion Start | 79,886,338 |
| Deletion End | 79,888,117 |
| Deletion Size | 1,780 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,886,554 | 79,886,573 | GCCTGAGGGTGGTTACTGTG |
| TUP | 79,886,574 | 79,886,600 | AAGGCACTGGGTCTCAAGTCTAAGGGC |
| TUR | 79,886,633 | 79,886,652 | GGCTGGCTAGGGAAACTGAG |
| TDF | 79,887,676 | 79,887,695 | GGGCACAAACAGACCATCAC |
| TDP | 79,887,696 | 79,887,724 | CCAGTGTGCTACAAGAGCTCAATGTGACA |
| TDR | 79,887,758 | 79,887,777 | CGTGGCACCAGAGTGCATAC |
PCR Primers
| Primer | Sequence |
| SU | GAAGATGGATGTCTGCTGAG |
| SD | CCTAGCTGAACATGGGATTTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12945A-C2 | VGB6 | C57BL/6NTac | |
| 12945A-D5 | VGB6 | C57BL/6NTac | |
| 12945A-F4 | VGB6 | C57BL/6NTac | |
| 12945A-G7 | VGB6 | C57BL/6NTac | |
