| Gene | Ndufb6 |
| Chromosome | 4 |
| Deletion Start | 40,279,334 |
| Deletion End | 40,270,762 |
| Deletion Size | 8,573 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,279,068 | 40,279,090 | CCACTCCTCTCAGTTCCATCAAG |
| TUP | 40,279,042 | 40,279,064 | CAACCCTGTCCAGAGCCCGCATA |
| TUR | 40,278,972 | 40,278,993 | AATAGCGACCTGACAGCGTCTC |
| TDF | 40,271,586 | 40,271,609 | GGCTTAACAGAACAAGTTTCCTTC |
| TDP | 40,271,544 | 40,271,573 | TAGACTAGTATGAGTCAGGCTGTCCTCCCA |
| TDR | 40,271,526 | 40,271,543 | GGGCTGGGACAAACAGAC |
PCR Primers
| Primer | Sequence |
| SU | ACTTCTCACACTTTCCAACAC |
| SD | AACACTTCACATCCTCATGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 119 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12949A-C2 | VGB6 | C57BL/6NTac | |
| 12949A-D8 | VGB6 | C57BL/6NTac | |
| 12949A-E12 | VGB6 | C57BL/6NTac | |
| 12949A-F10 | VGB6 | C57BL/6NTac | |
