| Gene | Asphd1 |
| Chromosome | 7 |
| Deletion Start | 126,948,373 |
| Deletion End | 126,946,040 |
| Deletion Size | 2,334 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,947,782 | 126,947,805 | CCAGTTGGCCTCAAACTCACTAAG |
| TUP | 126,947,755 | 126,947,778 | TGCCTCCCACTACCTCCTGAGTTC |
| TUR | 126,947,657 | 126,947,677 | GCCAGGTTGGTGCTATTGAAC |
| TDF | 126,946,664 | 126,946,685 | GACCAAAGACCTGGAGGGATTC |
| TDP | 126,946,640 | 126,946,663 | CAGCCAATCTGCTCTCAGCAGCAC |
| TDR | 126,946,562 | 126,946,583 | CACCCGGTAAAGAAGGCAGATG |
PCR Primers
| Primer | Sequence |
| SU | CTACCAGACCTGCCTTCGTC |
| SD | AGGTCTGCTGCTGACTCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 149 bp | + | + | - |
| TDF | TDR | 124 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12951A-B7 | VGB6 | C57BL/6NTac | |
| 12951A-D2 | VGB6 | C57BL/6NTac | |
| 12951A-D9 | VGB6 | C57BL/6NTac | |
| 12951A-E7 | VGB6 | C57BL/6NTac | |
