| Gene | Gm266 |
| Chromosome | 12 |
| Deletion Start | 111,485,767 |
| Deletion End | 111,485,089 |
| Deletion Size | 679 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 111,485,542 | 111,485,563 | GAGCATCTGGTCACCCTCAAAG |
| TUP | 111,485,516 | 111,485,538 | TGTACATCAGGAACAGCGACGGC |
| TUR | 111,485,488 | 111,485,506 | CGTCGCACACGCTGTAGAG |
| TDF | 111,485,172 | 111,485,190 | CCGCCGAGAGGTTCATTGG |
| TDP | 111,485,153 | 111,485,171 | CTAGCGCGCCACGGTCCGC |
| TDR | 111,485,091 | 111,485,113 | GCGGTGAATACACAGAAAAGCAG |
PCR Primers
| Primer | Sequence |
| SU | AGCTCAGGACTGACTGAGGG |
| SD | TTGTCTCCCGTCCAGTATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12952A-C9 | VGB6 | C57BL/6NTac | |
| 12952A-F6 | VGB6 | C57BL/6NTac | |
| 12952A-F12 | VGB6 | C57BL/6NTac | |
| 12952A-H5 | VGB6 | C57BL/6NTac | |
