| Gene | Gm1679 |
| Chromosome | 5 |
| Deletion Start | 110,354,183 |
| Deletion End | 110,355,910 |
| Deletion Size | 1,728 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,354,751 | 110,354,771 | CCTTGAATGCTGGGACCTGTG |
| TUP | 110,354,772 | 110,354,794 | CTGCCTCCCACTGAGCGAGAACA |
| TUR | 110,354,806 | 110,354,823 | GGTGCGCTTTGTGCAGTG |
| TDF | 110,355,429 | 110,355,453 | TCCTTTACTTTCTACTGGCCCTTAG |
| TDP | 110,355,458 | 110,355,486 | TCCAGAGGACAAAACCTCACAGAACAAGA |
| TDR | 110,355,488 | 110,355,509 | CACAAGCCCCTGTTCTCCATAG |
PCR Primers
| Primer | Sequence |
| SU | GCTCTCTTCCACTCTCCCCT |
| SD | AGGCACCAACATCTCAAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12956A-G3 | VGB6 | C57BL/6NTac | |
| 12956A-B9 | VGB6 | C57BL/6NTac | |
| 12956A-D5 | VGB6 | C57BL/6NTac | |
