| Gene | 4933407P14Rik |
| Chromosome | 11 |
| Deletion Start | 104,186,379 |
| Deletion End | 104,190,842 |
| Deletion Size | 4,464 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,187,118 | 104,187,141 | GGGAAGATGACGACTTTGAAGATG |
| TUP | 104,187,144 | 104,187,171 | CCTATGGATTTCACACCAGCCATGACGG |
| TUR | 104,187,180 | 104,187,198 | TGGAGCAGGACATGGTGAC |
| TDF | 104,189,954 | 104,189,974 | CTGACTGTCTACGCCTCTTTC |
| TDP | 104,189,976 | 104,190,000 | TGGAGTTTGGTCCGAGTAAACCAGC |
| TDR | 104,190,035 | 104,190,054 | TGCAGCCACTGTTTGCTAAG |
PCR Primers
| Primer | Sequence |
| SU | CCGGGCTAGCTGTACTGAGG |
| SD | GGAGCCCTTCAAACCCATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12958A-B9 | VGB6 | C57BL/6NTac | |
| 12958A-F6 | VGB6 | C57BL/6NTac | |
| 12958A-D1 | VGB6 | C57BL/6NTac | |
| 12958A-E7 | VGB6 | C57BL/6NTac | |
| 12958A-D4 | VGB6 | C57BL/6NTac | |
| 12958A-D3 | VGB6 | C57BL/6NTac | |
