| Gene | Ang6 |
| Chromosome | 14 |
| Deletion Start | 44,002,266 |
| Deletion End | 44,001,767 |
| Deletion Size | 500 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,002,136 | 44,002,153 | TGCCAAGCCAACAGGCTG |
| TUP | 44,002,105 | 44,002,124 | ACTGTTATCTGTGTATGATG |
| TUR | 44,002,068 | 44,002,093 | CTTCTTTGCATTTCAAGGTTTCTTGG |
| TDF | 44,001,991 | 44,002,010 | GAGGCCCTTATGGAGCAAAC |
| TDP | 44,001,954 | 44,001,982 | AAGCAATTCTCCCTTCCAGATCACCACTT |
| TDR | 43,962,807 | 43,962,825 | GCATGGAGGCTTGGGAGAC |
PCR Primers
| Primer | Sequence |
| SU | CGCCCTAGGATCTAGCACAG |
| SD | TCATCCTCAGCACCATAGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 39204 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12961A-G7 | VGB6 | C57BL/6NTac | |
| 12961A-C4 | VGB6 | C57BL/6NTac | |
| 12961A-E6 | VGB6 | C57BL/6NTac | |
| 12961A-B3 | VGB6 | C57BL/6NTac | |
