| Gene | 7420426K07Rik |
| Chromosome | 9 |
| Deletion Start | 98,903,287 |
| Deletion End | 98,903,946 |
| Deletion Size | 660 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,903,439 | 98,903,457 | CCCACTTGGGTCTGCTGTC |
| TUP | 98,903,459 | 98,903,486 | TGGACATGTGCTGGAAACTCATACGCAG |
| TUR | 98,903,502 | 98,903,522 | CTGTCTCTTACCGGAGCACTG |
| TDF | 98,903,653 | 98,903,674 | TCTCCTGTTGCTGAGAGTTGTG |
| TDP | 98,903,676 | 98,903,698 | TCCGTGGTTCACCTGGAGTCTCA |
| TDR | 98,903,717 | 98,903,737 | GCAGAGGTGAGTCTGGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | TCTGAAACCCAGATGTTCCC |
| SD | TTCAGGGCTGAACCATCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12963A-A11 | VGB6 | C57BL/6NTac | |
| 12963A-E1 | VGB6 | C57BL/6NTac | |
| 12963A-F5 | VGB6 | C57BL/6NTac | |
| 12963A-F6 | VGB6 | C57BL/6NTac | |
