| Gene | Timm8a2 |
| Chromosome | 14 |
| Deletion Start | 122,034,690 |
| Deletion End | 122,035,020 |
| Deletion Size | 331 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,034,745 | 122,034,764 | AGCGGTTCATGGAGGCAGAG |
| TUP | 122,034,768 | 122,034,792 | CAGAAGCAGAGGGTGCAGTTGCTCA |
| TUR | 122,034,810 | 122,034,830 | CATGCACTTCTCCCAGCAGAG |
| TDF | 122,034,892 | 122,034,914 | GCTTCATTGATACGAGCCAGTTC |
| TDP | 122,034,917 | 122,034,942 | CTTGAATCGACTGGAGCAGACCCAGA |
| TDR | 122,034,967 | 122,034,987 | GCTGAGATCAGTCGGAAAGTC |
PCR Primers
| Primer | Sequence |
| SU | ACCACCTTGTATGGCTTTGG |
| SD | GCAGCTCTTATTCATCTGTCAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12965A-B5 | VGB6 | C57BL/6NTac | |
| 12965A-D9 | VGB6 | C57BL/6NTac | |
| 12965A-C9 | VGB6 | C57BL/6NTac | |
| 12965A-G11 | VGB6 | C57BL/6NTac | |
