| Gene | Calhm1 |
| Chromosome | 19 |
| Deletion Start | 47,144,171 |
| Deletion End | 47,140,964 |
| Deletion Size | 3,208 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,143,809 | 47,143,827 | CACACTGCTGGATGGCAAG |
| TUP | 47,143,785 | 47,143,808 | TGCTTTCTCTGTGCCTTCTGCACA |
| TUR | 47,143,709 | 47,143,729 | CCAGTAGGCGAGCAAGTTCTG |
| TDF | 47,141,470 | 47,141,489 | CGTTCGTGGTACGCTCTGTG |
| TDP | 47,141,442 | 47,141,464 | CTGCTTCACGCAGGTCGCCTTTC |
| TDR | 47,141,397 | 47,141,419 | GCTTGCGCTCAATGTCAATGTAG |
PCR Primers
| Primer | Sequence |
| SU | TTGTCTGCACCTCTTCCTCC |
| SD | GCCCTCAGTTTCTTGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 119 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12970A-C4 | VGB6 | C57BL/6NTac | |
| 12970A-D12 | VGB6 | C57BL/6NTac | |
| 12970A-G3 | VGB6 | C57BL/6NTac | |
