| Gene | Chrne |
| Chromosome | 11 |
| Deletion Start | 70,619,172 |
| Deletion End | 70,614,877 |
| Deletion Size | 4,296 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,618,335 | 70,618,356 | GGTTGGTGAAGGAGGTTCAGAC |
| TUP | 70,618,299 | 70,618,328 | AAAGCTTGGTTTCCACAGTATTGATGGGCA |
| TUR | 70,618,276 | 70,618,293 | TGCTGTCGTAGGCCACTC |
| TDF | 70,615,405 | 70,615,422 | GCATCGGCACGGAACTTG |
| TDP | 70,615,379 | 70,615,402 | CCGGTAAGCACTGCTCTTGGGTCA |
| TDR | 70,615,354 | 70,615,374 | GTCCCTAACGGCGAGTGTATG |
PCR Primers
| Primer | Sequence |
| SU | GTAATATGGGAGGTGCGGG |
| SD | GCAGAGGGTAGAGGAAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12973A-A7 | VGB6 | C57BL/6NTac | |
| 12973A-A8 | VGB6 | C57BL/6NTac | |
| 12973A-B8 | VGB6 | C57BL/6NTac | |
| 12973A-E6 | VGB6 | C57BL/6NTac | |
