| Gene | Adra2a |
| Chromosome | 19 |
| Deletion Start | 54,046,218 |
| Deletion End | 54,047,635 |
| Deletion Size | 1,418 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,046,812 | 54,046,832 | GAGCCAAGCTGCAAGATCAAC |
| TUP | 54,046,842 | 54,046,868 | TGGTATGTCATCTCCTCGTCCATCGGT |
| TUR | 54,046,871 | 54,046,888 | AGGCAAGGCGCGAAGAAG |
| TDF | 54,047,536 | 54,047,558 | ACACCATCTTCAACCACGACTTC |
| TDP | 54,047,559 | 54,047,582 | CGACGCGCCTTCAAGAAGATCCTC |
| TDR | 54,047,607 | 54,047,625 | AAGGCCCATGCGCTCACAC |
PCR Primers
| Primer | Sequence |
| SU | AGACTTCATGCTTTCGCTCAG |
| SD | TTCATTAACCCTTTGGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12974A-D10 | VGB6 | C57BL/6NTac | |
| 12974A-D6 | VGB6 | C57BL/6NTac | |
