| Gene | Adra2b |
| Chromosome | 2 |
| Deletion Start | 127,363,568 |
| Deletion End | 127,364,991 |
| Deletion Size | 1,424 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,363,939 | 127,363,958 | CAACTCCAAGCGCACTCCAC |
| TUP | 127,363,960 | 127,363,986 | CCGCATCAAATGCATCATCCTCACCGT |
| TUR | 127,363,991 | 127,364,013 | AGAGAAATGACGGCTGCAATGAG |
| TDF | 127,364,475 | 127,364,498 | AAGAGGATGAAGAGGAGGTGGAAG |
| TDP | 127,364,510 | 127,364,533 | CAAACACTGCCAGCTTCTCCTGCC |
| TDR | 127,364,535 | 127,364,555 | GCAAGGGTGGGTTGAATACTG |
PCR Primers
| Primer | Sequence |
| SU | CCAGTCGGTTCTGTGAGTCC |
| SD | AGCCTGCCTGTTGGCTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12975A-B7 | VGB6 | C57BL/6NTac | |
| 12975A-D1 | VGB6 | C57BL/6NTac | |
| 12975A-E1 | VGB6 | C57BL/6NTac | |
| 12975A-G10 | VGB6 | C57BL/6NTac | |
